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血液细胞重编程iPS细胞建系-诺为生物,eBioscience,STEMCELL, SunJinLab,Lucigen,Novobiotec,Biosearch,iBiochips授权代理,干细胞,免疫学研究平台

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血液细胞重编程iPS细胞建系

hiPS细胞建系

专用培养基  个性化服务  7×24h技术支持

为您实现将疾病模型培养在培养皿中的理想

Generation of Integration-Free iPS Cells From 5ml Human Blood


如何取的合适的样本?

如何防止样本被污染?

实验室实验条件是否能够满足?

如何高效率重编程生成hiPS细胞?

          。。。。。。。。。。???

还在为取材困难而无法建立疾病模型烦恼吗?我们可利用Episomal  iPSC Reprogramming高效率重编程系统将5-10 mL外周血经转染后即可诱导生成无外源基因插入整合的hiPSCs,为您轻松建立理想的疾病模型,用于iPSCs疾病模型的建立、药物开发、细胞治疗以及其它再生医学研究领域。


适合应用领域:

  1. iPS疾病模型的建立

  2. 创伤样本无法取材

  3. 疾病模型用于药物开发

  4. 细胞治疗以及再生医学研究

  5. 尿液样本量少,无法满足实验需求

from blood to iPS.png


取材简便:5-15 mL血即可完成重编程建系。

实验周期短:从人外周血到iPS细胞系建成只需3-4周。

无基因整合:采用Episomal系统,无外源基因插入整合。

重编程效率高:重编程效率高,提供完整的iPS检测报告。

hiPSCs细胞鉴定:

iPS.png


载体信息:

pEV-SFFV-OCT4-Wpre; 

pEV-SFFV-SOX2-Wpre; 

pEV-SFFV-Myc-Wpre; 

pEV-SFFV-KLF4-Wpre; 

pEV-SFFV-OCT4-E2A-SOX2-Wpre;

pEV-SFFV-MYC-E2A-KLF4-Wpre; 

pEV-SFFV-BCL-XL-Wpre


注:

SFFV: spleen focus-forming virus U3 promoter; 

 E2A: a self-cleavage site derived from equine rhinitis A virus,(CAG TGT ACT AAT TAT GCT CTC TTG AAA TTG GCT GGA GAT GTT GAG AGC AAC CCA GGT CCC); 

WPRE: posttranscriptional regulatory element; Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed, creates a tertiary structure enhancing expression. The sequence is commonly used in molecular biology to increase expression of genes delivered by viral vectors.

SV40PolyA: polyadenylation signal from SV40 virus; 

OriP: EBV origin of replication; 

EBNA1: Epstein–Barr nuclear antigen 1, which plays essential roles in replication and persistence of episomal plasmid in infected cells.



请联系诺为生物,www.novobiotec.com    support@nwbiotec.com  电话:010-57438396

 

参考文献:

1. Meng X, Neises A, Su RJ, Payne KJ, Ritter L, Gridley DS, Wang J, Sheng M, Lau KH, Baylink DJ, Zhang XB: Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Molecular Therapy, 2012;20(2):408-16.


2. Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N, Kearns-Jonker M, Gridley DS, Wang J, Lau KHW, Zhang XB: Efficient generation of integration-free iPS cells from human adult peripheral blood using BCL-XL together with Yamanaka factors. PLoS One. 2013, 8(5): e64496.


3. Zhang XB: Cellular reprogramming of human peripheral blood cells. Genomics Proteomics Bioinformatics. 2013 Oct;11(5):264-74.


4. Su RJ, Neises A, Zhang XB: Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors. Methods Mol Biol. 2016;1357:57-69.


5. Wen W#, Zhang JP#, Xu J#, Su RJ, Neises A, Ji GZ, Yuan W, Cheng T*, Zhang XB*. Enhanced generation of integration-free iPSCs from human adult peripheral blood mononuclear cells with an optimal combination of episomal vectors. Stem Cell Reports. 2016 Jun 14;6(6):873-84.


6. Wen W, Zhang JP, Chen W, Arakaki C, Li XL, Baylink D, Botimer GD, Xu J, Yuan W, Cheng T*, Zhang XB*. Generation of integration-free induced pluripotent stem cells from human peripheral blood mononuclear cells using episomal vectors. J Vis Exp. 2017 Jan 1;(119). doi: 10.3791/55091.


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